Guayaquil Day is observed in Ecuador's largest city, Santiago de Guayaquil, every July 24. This regional holiday commemorates the foundation of the city, which is officially recognized as having been established in July 1538 by Spanish conquistador Francisco de Orellana, although a native village existed prior to this settlement. Guayaquil played a significant role in the Spanish American wars of independence, notably hosting the Guayaquil Conference on July 26, 1822, where Simon Bolivar and Jose de San Martin discussed the future of Peru and other former Spanish colonies. Guayaquil Day is an official holiday, distinct from Guayaquil Independence Day, which marks the beginning of the Ecuadorian War of Independence. The day is celebrated with various local festivities, reflecting the city's rich history and cultural significance.